Structure and evolution-guided design of minimal RNA-guided nucleases

Petr Skopintsev*, Isabel Esain Garcia*, Evan C. DeTurk*, Peter H. Yoon, Zehan Zhou, Trevor Weiss, Maris Kamalu, Ajit Chamraj, Kenneth J. Loi, Conner J. Langeberg, Ron S. Boger, Hunter Nisonoff, Hannah M. Karp, LinXing Chen, Honglue Shi, Kamakshi Vohra, Jillian F. Banfield, Jamie H.D. Cate, Steven E. Jacobsen, Jennifer A. Doudna. Science 393, no. 6808 (2026): 313-318. doi.org/10.1126/science.aed6123.

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Stepwise DNA unwinding gates TnpB genome-editing activity

Zehan Zhou*, Iren Saffarian-Deemyad*, Honglue Shi*, Trevor Weiss*, Kamakshi Vohra, Petr Skopintsev, Peter H. Yoon, Marena I. Trinidad, Conner J. Langeberg, Maris Kamalu, Jasmine Amerasekera, Erin E. Doherty, Kevin D.P. Aris, Noor Al-Sayyad, Brittney W. Thornton, Rachel F. Weissman, Kevin M. Wasko, Isabel Esain-Garcia, Evan C. DeTurk, David F. Savage, Steven E. Jacobsen, Zev Bryant, Jennifer A. Doudna. bioRxiv (2026). https://www.biorxiv.org/content/10.64898/2026.01.09.698545v2. 

Eukaryotic RNA-guided endonucleases evolved from a unique clade of bacterial enzymes

Peter H. Yoon*, Petr Skopintsev*, Honglue Shi*, LinXing Chen, Benjamin A. Adler, Muntathar Al-Shimary, Rory J. Craig, Kenneth J. Loi, Evan C. DeTurk, Zheng Li, Jasmine Amerasekera, Marena Trinidad, Hunter Nisonoff, Kai Chen, Arushi Lahiri, Ron Boger, Steve Jacobsen, Jillian F. Banfield, Jennifer A. Doudna. Nucleic Acids Research 51, no. 22 (2023): 12414-12427. doi.org/10.1093/nar/gkad1053. 

* = equal contribution